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ksgA [2019-01-30 22:37:57]
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ksgA [2019-01-30 22:37:57]

rRNA adenine dimethyltransferase
locus
BSU00420
pI
5.71
mw
32.57 kDa
protein length
292 aa Sequence Blast
gene length
879 bp Sequence Blast
function
rRNA maturation
product
rRNA adenine dimethyltransferase
essential
no
ec
2.1.1.-
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.2|rRNA modification and maturation]

    • Gene

    Coordinates
    50,640 → 51,518

    The protein

    Catalyzed reaction/ biological activity

  • methylation of A1518 and A1519 in 16S rRNA

    • Protein family

  • [SW|Methyltransferase superfamily] (according to UniProt)

    • Structure

  • [PDB|3FUU] (from ''Thermus thermophilus'', 35% identity) [Pubmed|19285505]

    • Expression and Regulation

    Operons

    genes
    [gene|5BA7AA691CFE4635DEFBD0B8B820F487B58A5DB7|rnmV]-[gene|FF329359CBE52C75A39237E8A6BB56F6AB321AFB|ksgA]
    description
    [Pubmed|11233981]
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    Biological materials

    Mutant

  • MGNA-B907 (ksgA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1906 NBRP B. subtilis, Japan]
  • BKE00420 (Δ[gene|FF329359CBE52C75A39237E8A6BB56F6AB321AFB|ksgA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE00420 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CTTTATTCATTCTGTTCCTC, downstream forward: _UP4_TAAAAGGGCTTTTTGTTTTG
  • BKK00420 (Δ[gene|FF329359CBE52C75A39237E8A6BB56F6AB321AFB|ksgA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK00420 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CTTTATTCATTCTGTTCCTC, downstream forward: _UP4_TAAAAGGGCTTTTTGTTTTG

    • References

  • 16497325,19001112,11233981,19285505